Structure of a polysaccharide from the seeds of Cassia tora. Part II. Partial acidic hydrolysis

Abstract

Partial acidic hydrolysis of the polysaccharide obtained from the seeds of Cassia tora Linn. gave the following oligosaccharides, which were characterized on the basis of chemical and physical constants: 4-O-β-D-glucopyranosyl-D-mannopyranose (0.83%), 4-O-β-D-mannopyanosyl-D-mannopyranose (5.17%), 4-O-β-D-glucopyranosyl-D-glucopyranose (0.48%), 6-O-β-D-glucopyranosyl-D-glucopyranose (0.47%), 6-O-α-D-galactopyranosyl-D-mannopyranose (3.98%), O-β-D-glucopyranosyl-(1→6)-O-β-D-glucopyranosyl-(1→6)-D-glucopyranose (0.57%), O-α-D-xylopyranosyl-(1→6)-O-β-D-glucopyanosyl-(1→4)D-glucopyranose (1.05%), O-β-D-glucopyranosyl-(1→4)-O-β-D-glucopyranosyl-(1→4)-D-mannopyranose (0.70%), O-β-D-glucopyranosyl-(1→4)-O-β-D-mannopyranosyl(1→4)-D-mannopyranose (0.78%), O-β-D-mannopyranosyl-(1→4)-O-β-D-glucopyranosyl-(1→4)-D-glucopyranose (0.52%), O-α-D-galactopyranosyl-(1→6)-O-[β-D-mannopyranosyl-(1→4)]-D-mannopyranose (2.58%), O-β-D-mannopyranosyl-(1→4)-O-β-D-mannopyranosyl-(1→4)-D-glucopyranose (1.50%), O-β-D-glucopyranosyl-(1→4)-O-β-D-glucopyransoyl-(1→4)-D-glucopyranose (0.63%), O-β-D-mannopyanosyl-(1→4)-O-β-D-mannopyranosyl-(1→4)-D-mannopyranose (7.08%), O-α-D-galactopyranosyl-(1→6)-O-β-D-mannopyranosyl-(1→4)-D-mannopyranose (5.25%), O-β-D-glucopyranosyl-(1→6)-O-β-D-glucopyranosyl-(1→6)-O-β-D-glucopyranosyl-(1→4)-D-mannopyranose (2.33%),O-β-D-glucopyranosyl-(1→4)-O-β-D-glucopyranosyl-(1→4)-O-β-D-glucopyranosyl-(1→4)-D-glucopyranose (0.67%), O-β-D-glucopyranosyl-(1→[4-O-β-D-glucopyranosyl-1]₃→4)-D-glucopyranose (0.79%), O-β-D-glucopyranosyl-(1→[4-O-β-D-glucopyranosyl-1]₄→4)-D-glucopyranose (0.92%), and O-β-D-mannopyranosyl-(1→4)-O-β-D-glucopyranosyl-(1→[4O-β-D-glucopyranosyl-1]₃→4)-D-glucopyranose (2.46%). Efforts have also been made to ascertain the pattern of hydroysis from studies of degraded polysaccharides isolated at various intervals during partial acidic hydrolysis.