1-Cyano-4-dimethylaminopyridinium perchloroate or fluoroborate rapidly react with thiols in neutral or acidic medium: in 11 min at pH 3·6, 98% of the catalytic activity of papain is inhibited; cysteine residues nos. 7 and 19 of the reduced B-chain of bovine insulin are quantitatively cyanylated at pH 3·5, then the N-peptide bonds are selectively cleaved at pH 9·5.
M. Wakselman, E. Guibé-Jampel, A. Raoult and W. D. Busse, J. Chem. Soc., Chem. Commun., 1976, 21 DOI: 10.1039/C39760000021
To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.
If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.
If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.
Read more about how to correctly acknowledge RSC content.
Please wait while we load your content...