The gas-chromatographic determination of selenium(VI) and total selenium in milk, milk products and albumin with 1,2-diamino-4-nitrobenzene

Abstract

Selenium(IV) reacts with 1,2-diamino-4-nitrobenzene to form 5-nitropiazselenol, which is detected by means of a gas chromatograph equipped with an electron-capture detector. Trace amounts of selenium(VI) and total selenium in milk, milk products and albumin were determined by this method with a practical detection limit of 0.005 µg. The amount of selenium(VI) found in milk was about 60% of the total selenium content.