The isomerization and cyclization of all-trans-farnesyl pyrophosphate (1) to trichodiene (2) by a cellfree system obtained from Trichothecium roseum, takes place with the loss of a pro-1(S) hydrogen atom of farnesyl pyrophosphate and its ultimate replacement with a pro-4(S)-hydrogen atom of NADPH.
R. Evans and J. R. Hanson, J. Chem. Soc., Chem. Commun., 1975, 231b DOI: 10.1039/C3975000231B
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