An enzymic method for the determination of [1-14C]lactose
Abstract
A simple, rapid and specific method for the determination of [1-14C]lactose in biological fluids is described. It is based on the enzymic removal of the 1-14C atom of lactose as [14C]carbon dioxide, using commercially available enzymes. The assay involves only one critical addition and the entire reaction can be carried out in a scintillation vial.