Gel supports for electrophoresis with pyridine-acetic acid media

Abstract

Agar gels that are suitable for use with an 80 per cent. solution of pyridine in water and cross-linked polystyrene gels in pyridine-acetic acid are effective support media for electrophoresis. They provide the means for widening the application of electrophoresis to materials themselves insoluble in aqueous media but soluble in pyridine-acetic acid.

Illustrative examples of the electrophoresis of methylene blue, rhodamine B, alizarin red, alizarin blue and eosin are given.