The C-24 hydrogen atom of the Δ24 precursor is eliminated during biosynthesis of Stigmasterol in tissue cultures of Nicotiana tabaccum and Dioscorea tokoro. Thus the Δ24(25) intermediate is involved in Stigmasterol biosynthesis.
Y. Tomita and A. Uomori, J. Chem. Soc., Perkin Trans. 1, 1973, 2656 DOI: 10.1039/P19730002656
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