Determination of cyclamate in soft drinks by reaction with nitrous acid. Part II. Manual and semi-automated methods: determination of cyclohexyl nitrite by diazotisation and coupling with Bratton-Marshall reagent

Abstract

The determination of cyclamate at a concentration of approximately 1 mg ml^–1 in soft drinks is described. Cyclohexyl nitrite, derived from cyclamate by reaction of the latter with nitrous acid, is determined in a non-aqueous system by initial diazotisation with sulphanilamide and subsequent coupling with 2-aminoethyl-1-naphthylamine. Manual and semi-automated procedures have been evaluated and interference has been studied.