C.d. studies of C-glycosylflavones have shown that a positive Cotton effect at 250–275 nm indicates that the glycosyl residue is linked to C-6 (e.g. isovitexin) while a negative Cotton effect at 250–275 nm indicates it is linked to C-8 (e.g. vitexin).
W. Gaffield and R. M. Horowitz, J. Chem. Soc., Chem. Commun., 1972, 648 DOI: 10.1039/C39720000648
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