Spectrophotometric determination of diosgenin in Dioscorea composita following thin-layer chromatography

Abstract

A rapid and accurate method is described for the determination of diosgenin in Dioscorea composita roots. After acid hydrolysis and solvent extraction of the root, pure diosgenin is isolated by thin-layer chromatography, treated with sulphuric acid-methanol, and the resultant chromophore is measured by spectrophotometry.

Similarly, gas-liquid chromatography is used to evaluate statistically the accuracy of the method.