Effect of m-cresol on the interaction of Biebrich Scarlet with a-chymotrypsin

Abstract

With a view to explaining the effect of m-cresol on the kinetics of dye uptake by wool and other proteins, a study has been made of the interaction of Biebrich Scarlet (C.I. Acid Red 66) with α-chymotrypsin in the presence and absence of m-cresol. It has been found that a 1:1 dye-protein complex is formed in the absence of m-cresol and that m-cresol displaces the dye from the complex through allosteric competition.