Analysis of steroids. Part XVII. A differential spectrophotometric variant of the diethyl oxalate method for the determination of ketosteroid contaminants

Abstract

A method that consists of spectrophotometry following a Claisen condensation with diethyl oxalate was used for the determination of ketosteroid contaminants. Absorption of the main steroid component was eliminated by a differential spectrophotometric procedure. The proposed method is useful for the determination of ketosteroid contaminants present at the level of 0·1 per cent.