A reported trapping of a putative acyl–pepsin intermediate in the pepsin-catalysed hydrolysis of dipeptide substrates using radioactive methanol cannot be confirmed, and the intermediacy of an acyl–enzyme therefore remains in doubt.
T. M. Kitson and J. R. Knowles, J. Chem. Soc. D, 1970, 361b DOI: 10.1039/C2970000361B
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