Evaluation of esterases from livers of beef, pig, sheep, monkey and chicken for detection of some pesticides by thin-layer chromatographic-enzyme inhibition technique

Abstract

The differences in the detection of eight pesticides with extracts of beef, sheep, pig, monkey and chicken livers are described. Dichlorvos, ethion and dimethoxon, with or without bromine exposure, were detected with the 2000 or 3000-g supernatants of the water or tris extracts of the livers. After bromination, carbaryl, oxydemeton-methyl, demeton and the thiol isomer of demeton sulfone were detected with beef or sheep liver extracts. Oxydemeton-methyl and the thiol isomer of demeton sulfone were detected with pig or monkey liver extracts after bromination; however, demeton was detected with or without bromination. Carbaryl was detected with or without bromination with the 2000 or 3000-g supernatants of extracts of pig livers. Carbaryl was not detected with the water extracts of monkey liver, but it was detected with the tris extracts of monkey livers after bromination. Either of the extracts of chicken livers detected carbaryl after bromine exposure; the demeton compounds were not detected.

In general, the 2000 g supernatants had higher protein contents and gave more intense thin-layer chromatographic backgrounds than the 3000 g super-natants. Tris and water extracts gave comparable background intensity, although the former had a protein content lower than that of the latter.