Efficiency of extraction of metabolically incorporated HEOD (carbon-14) from pheasant tissues, eggs and faeces

Abstract

Acetonitrile partitioning and Florisil column procedures were examined for efficiency of extraction and purification of HEOD (carbon-14) residues. Fat, liver, eggs and faeces from pheasant hens fed with 1·5 mg of HEOD (carbon-14) were used. Gas-liquid chromatographic analysis of HEOD-fortified controls gave results similar to those obtained by liquid scintillation counting when samples were extracted and purified with a Florisil column. Analysis by liquid scintillation counting showed that recoveries of HEOD (carbon-14) from fortified controls compared favourably with recoveries of HEOD (carbon-14) that had been metabolically incorporated into the tissue for both the partitioning and Florisil column procedures. It was concluded that gas-liquid chromatographic analysis of a fortified control sample gave an accurate measurement of the recovery of HEOD from tissues in which the HEOD was metabolically incorporated within the range of concentrations studied.