Issue 46, 2018

Immunomagnetic bead-based biotin-streptavidin system for highly efficient detection of aflatoxin B1 in agricultural products

Abstract

The potential homogeneous assay employing immunomagnetic beads (IMB) has been receiving attention as a screening tool in food-safety control; the method is simple, efficient, and does not require long incubation times or complex separation steps. In this study, a homogeneous immunoassay has been successfully developed and applied in the determination of aflatoxin B1 (AFB1) contamination in agricultural products by coupling IMB and the biotin-streptavidin (BSA) (BSA-IMB) system. Under optimal conditions, the limit of detection (LOD, IC10), half-maximal inhibition concentration (IC50) and detection range (IC20–IC80) of BSA-IMB are 0.00579, 0.573 and 0.0183–17.9 ng mL−1, respectively, for AFB1. The detection of AFB1 by BSA-IMB can be achieved in 40 min (ELISA needs at least 180 min). The cross-reactivities of BSA-IMB with its analogues are negligible (<3.82%); these results indicate high selectivity. The spiked recoveries are in the range from 89.6 to 118.2% with relative standard deviations (RSDs) of 3.4 to 13.2% for AFB1 in agricultural product samples. Furthermore, the results of BSA-IMB for authentic samples show reliability and high correlation of 0.9928 with an HPLC-fluorescence detector. The proposed BSA-IMB system is demonstrated to be a satisfactory tool for homogeneous, efficient, sensitive, and alternative detection of AFB1 in a wide detection range for agricultural product samples.

Graphical abstract: Immunomagnetic bead-based biotin-streptavidin system for highly efficient detection of aflatoxin B1 in agricultural products

Supplementary files

Article information

Article type
Paper
Submitted
25 May 2018
Accepted
01 Jul 2018
First published
19 Jul 2018
This article is Open Access
Creative Commons BY-NC license

RSC Adv., 2018,8, 26029-26035

Immunomagnetic bead-based biotin-streptavidin system for highly efficient detection of aflatoxin B1 in agricultural products

M. Li, Y. Zhang, R. Zhao, Z. Liu, X. Hong, Y. Cui, Y. Xue and D. Du, RSC Adv., 2018, 8, 26029 DOI: 10.1039/C8RA04460K

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