Themed collection Lab on a Chip Recent HOT Articles

How can microfluidics address the significant limitations to the current tools that measure gene expression in single-animal studies?

This review focuses on the applications of organic transistors in cellular interfacing. It offers a comprehensive retrospective of the past, an overview of the latest innovations, and a glance on the future perspectives of this fast-evolving field.

Immunotherapy is a powerful therapeutic approach able to re-educate the immune system to fight cancer. In this scenario, tumor-on-chip can help to choose the right immunotherapeutic treatment.

Review of chip designs and data analytics to stratify heterogeneity in cellular systems via microfluidic impedance cytometry.

Active and passive techniques for bubble trapping and control in various microfluidic applications.

Acoustic induced nanoparticle patterning and location migration in inhomogeneous media formed in situ.

We present an accessible strategy for printing multi-material 3D nanostructured components via microfluidic two-photon direct laser writing.

Volumetric optical microscopy approaches that enable acquisition of three-dimensional (3D) information from a biological sample are attractive for numerous non-invasive imaging applications.

A microfluidics-enabled strategy for the controllable synthesis of immunomagnetic nanomaterials was developed, and the shape-dependent screening efficiency of CTCs was investigated.

This study presented a convenient method of gathering, splitting, merging, and sorting microdroplets by dynamic pneumatic rails in double-layered microfluidic devices.

A microfluidic device to quantitate antibody reactivities to SARS-CoV-2 antigens: spike protein, RDB, S1 subunit, and nucleocapsid, from 50 serum samples.

Isolation and identification of bacteria from human blood were automated on an integrated microfluidic system for sepsis-inducing bacteria.

A capillary and centrifuge-based rapid antimicrobial susceptibility testing system is developed to reduce the time of loading the sample and culture media while achieving a high-throughput testing capacity.

An integrated revolving needle emulsion generator (RNEG) is developed to achieve high-throughput production of monodispersed droplets in an off-chip manner.

We introduce a microfluidic Hele-Shaw trapping cell that enables the fabrication of hydrogel sheets whose composition can be abruptly and controllably changed.

An elastomeric composite material with skin-like mechanical responses and spatially heterogeneous rigidity is developed to accommodate stretchable electronic circuit systems.

The drug susceptibility of leukemia cells in whole blood is evaluated by using extreme-throughput imaging flow cytometry with deep learning.

Modular platform providing the building blocks to translate specialised microfluidics from the laboratory to a clinical environment.

The use of microfluidic devices may allow standardizing corona formation protocols and comparing data from different laboratories.

A gold bowtie nanoantenna integrated with a 30 nm × 30 nm nanochannel for single particle detection and counting in real time.

The diamagnetic repulsion of polymer microspheres in a microfluidic platform enables the discrimination of magnetic susceptibility of subtle paramagnetic solutions.

An implantable nanofluidic device for remote controlled drug delivery.

Selection of high DNA integrity sperm via planar swimming in a microfluidic device outperforms clinically established sperm preparation.

Controlled co-precipitation of biocompatible colorant-loaded nanoparticles in microfluidic channels for natural color drinks.

A one-mask microfabrication process to build artificial vascular networks for the analysis of blood flows and hemophysics.

Microfluidics and MALDI-TOF MS is a rapid, high-throughput, and accurate method for the identification of beer spoilage bacteria.

When PhC barcodes with stable characteristic reflection peaks are integrated with a superhydrophobic substrate they can realize the ultrasensitive multiple detection by the enrichment of the solution upon evaporation. Thus, the detection of miRNA was carried out by the system and it was found the detection limit was improved by about three orders.

We studied the dsDNA trajectories being advected while diffusing in a pressure driven flow in a nanoDLD array.

Microfluidic system integrated with cell collectives and traction microscopy demonstrates that collective cell migration plays a central role in development, regeneration, and metastasis.

We report a proof-of-principle demonstration of particle concentration to achieve high-throughput resistive pulse detections of bacteria using a microfluidic-channel-integrated micropore.

A new label-free and passive microfluidic technique to select cells based on single-cell glycolytic activity. The method has broad applicability and we demonstrate here single-cell droplet encapsulation and selection of live cells.

Surface acoustic wave (SAW) atomisation is investigated in the context of cryo electron microscopy grid preparation. Here, the primary requirements are a reproducible and narrow plume of droplets delivering a low fluid flow rate.

A mesofluidic device allows alignment, immobilization, compression, imaging, and recovery of hundreds of live Drosophila embryos to investigate mechanotransduction.

An acoustofluidic chip can liquefy stool samples in a continuous flow.

We present a methodology for building biologically inspired, soft microelectromechanical systems (MEMS) devices.

Latchable microfluidic valve arrays taking advantage of the shape memory polymer's multiple stable shapes and large change in stiffness for a small temperature variation.

A plastic-based acoustofluidic device for high-throughput platelet separation consisting of a transducer and a disposable chip.

We report here a 96 well-plate based pumpless platform for high-throughput dynamic multicellular culture and chemosensitivity evaluation.

Production of hydrocarbons from shale is a complex process that necessitates the extraction of multi-component hydrocarbons trapped in multi-scale nanopores.

We present a dextrous microfluidic device which features a reaction chamber with volume flexibility and acoustic mixing.

A microfluidic device for the co-cultivation of microbial cells.

Detection of AR-V7 in urinary EVs provides a simple and promising liquid biopsy tool for patients with prostate cancer.

Post-synthesis functionalized hydrogel microparticles were demonstrated in multiplex immunoassays with high sensitivity, a wide assay range, and fast detection.

Single-molecule detection schemes offer powerful means to overcome static and dynamic heterogeneity inherent to complex samples.

Bead-triggered breakup of a fluid jet into monodispersed droplets improves the throughput of bead-based droplet workflows, enabling the analysis of large populations and the detection of rare events.

On-demand two-phase encapsulation of electrokinetically separated analytes decouples electrokinetic separations from downstream analytical processes.

We report a novel strategy for bridging information transfer between electronics and biological systems within microdevices.

Overcoming solubility of membrane protein transmembrane domains to study their specificity to lipid compositions.

Replicating mechanical stress using two novel devices revealed that mechanical stress in retinal pigment epithelial cells induces choroidal neovascularization.

Microfluidic gradient generators are used to study the movement of living cells, lipid vesicles, and colloidal particles in response to spatial variations in their local chemical environment.

Single cell coupled proteomic and transcriptomic measurements are captured on a scalable microfluidic device.

Microfluidic culture has the potential to revolutionize cancer diagnosis and therapy.

This work presents a microfluidic device to challenge the deformability of normal and pathological red cells and their resistance to lysis upon mechanical stress.

We introduce a new platform for mechanobiology based on active substrates, made of Fe-coated polymeric micropillars, capable to apply mechanical stimuli with tunable spatio-temporal profile on a cell culture.

A fully automated droplet generation and analysis device based on pressure driven push-up valves for precise pumping of fluid and volumetric metering has been developed for high resolution hormone secretion sampling and measurement.

A novel microfluidic platform for extracellular vesicle extraction, microRNA isolation and detection with field-effect transistors for early detection of cardiovascular diseases.

Here we present a water-in-air droplet platform for micro-compartmentalization for single molecule guided synthesis and analysis consisting of a flow-system hosting dense arrays of aqueous microdroplets on a glass surface surrounded by air.

Microphysiological tissue engineering models of human skeletal muscle (myobundles) in series with a calibrated elastic membrane provide a platform to investigate the mechanism of muscle diseases and to study the response to drugs and toxins in vitro.

Tissues are increasingly being analyzed at this single cell level. We present a simple and inexpensive microfluidic filter device that can rapidly and effectively improve the quality of single cell suspensions obtained from digested tissue samples.

UniChip enables recirculating unidirectional perfusion with gravity-driven flow, facilitating reliable and cost-effective integration of shear stress-sensitive tissues into microphysiological systems.

To improve predictive efficacy of organ-on-a-chip devices, developers must consider cell heterogeneity.

This platform utilizes functionalized hydrogel posts contained within isolated nanoliter well reactors for quantitative and multiplex microRNA assays directly from unprocessed cell samples without needing prior nucleic acid extraction.

NemaFlex measures a behavior- and gait-independent measure of C. elegans strength analogous to maximum voluntary force in humans.

A simple and fast multi-scale microliquid patterning on an open surface is demonstrated.

A system for perfusing and interacting tumor fragments and immune cells and testing drug response with image analytics is reported.

Single CTC sequencing workflow using biophysical enrichment and single cell isolation by laser capture microdissection.

Recently, much effort has been focused on developing three-dimensional, paper-based microfluidic analytical devices (3D-μPADs) targeting in vitro diagnostics.

We introduce a multiparameter intrinsic cytometry approach for single-cell characterization that combines ≥2 label-free measurement techniques onto the same platform.

We present an injection molded lab-on-a-disc enabling quantification of bacterial metabolites in E. coli supernatant.

The application of soft lithography to create reaction–diffusion networks capable of generating spatiotemporal patterns analogous to biological central pattern generators.