Issue 16, 2022

Reversible inhibition of the oxidase-like activity of Fe single-atom nanozymes for drug detection

Abstract

Mechanism research of nanozymes has always been of great interest since their emergence as outstanding mimics of friable natural enzymes. An important but rarely mentioned issue in mechanism research of nanozymology is the inhibitory effect of nanozymes. And conventional nanozymes with various active sites hinder the mechanism research, while single-atom Fe–N–C nanozymes with similar active sites to natural enzymes exhibit structural advantages. Herein, we synthesized Fe single-atom nanozymes (Fe-SANs) with ultrahigh oxidase-like activity and found that a common analgesic-antipyretic drug 4-acetamidophenol (AMP) had inhibitory effects for the oxidase-like activity of Fe-SANs. We investigated the inhibitory effects in detail and demonstrated that the inhibition type was reversible mixed-inhibition with inhibition constants (Ki and Image ID:d2sc00212d-t1.gif) of 0.431 mM and 0.279 mM, respectively. Furthermore, we put forward a colorimetric method for AMP detection based on nanozyme inhibition. The research on the inhibitory effects of small molecules on nanozymes expands the scope of analysis based on nanozymes and the inhibition mechanism study may offer some insight into investigating the interaction between nanozymes and inhibitors.

Graphical abstract: Reversible inhibition of the oxidase-like activity of Fe single-atom nanozymes for drug detection

Supplementary files

Article information

Article type
Edge Article
Submitted
12 Jan 2022
Accepted
19 Mar 2022
First published
24 Mar 2022
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY-NC license

Chem. Sci., 2022,13, 4566-4572

Reversible inhibition of the oxidase-like activity of Fe single-atom nanozymes for drug detection

W. Wu, L. Huang, X. Zhu, J. Chen, D. Chao, M. Li, S. Wu and S. Dong, Chem. Sci., 2022, 13, 4566 DOI: 10.1039/D2SC00212D

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