Dynamic contrast with reversibly photoswitchable fluorescent labels for imaging living cells

Raja Chouket; Agnès Pellissier-Tanon; Annie Lemarchand; Agathe Espagne; Thomas Le Saux; Ludovic Jullien

doi:10.1039/D0SC00182A

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Dynamic contrast with reversibly photoswitchable fluorescent labels for imaging living cells

Raja Chouket,†^a Agnès Pellissier-Tanon,†^a Annie Lemarchand,

^b Agathe Espagne,

^a Thomas Le Saux^a and Ludovic Jullien

*^a

Author affiliations

* Corresponding authors

^a PASTEUR, Département de Chimie, École Normale Supérieure, PSL University, Sorbonne Université, CNRS, 24, rue Lhomond, 75005 Paris, France
E-mail: Ludovic.Jullien@ens.fr
Tel: +33 4432 3333

^b Sorbonne Université, Centre National de la Recherche Scientifique (CNRS), Laboratoire de Physique Théorique de la Matière Condensée (LPTMC), 4 Place Jussieu, Case Courrier 121, 75252 Paris Cedex 05, France

Abstract

Interrogating living cells requires sensitive imaging of a large number of components in real time. The state-of-the-art of multiplexed imaging is usually limited to a few components. This review reports on the promise and the challenges of dynamic contrast to overcome this limitation.

This article is part of the themed collection: Advances in Optical and Electrochemical Techniques for Biomedical Imaging

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Article information

DOI: https://doi.org/10.1039/D0SC00182A
Article type: Minireview
Submitted: 10 Jan 2020
Accepted: 23 Feb 2020
First published: 25 Feb 2020
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry

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Chem. Sci., 2020,11, 2882-2887

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Dynamic contrast with reversibly photoswitchable fluorescent labels for imaging living cells

R. Chouket, A. Pellissier-Tanon, A. Lemarchand, A. Espagne, T. Le Saux and L. Jullien, Chem. Sci., 2020, 11, 2882 DOI: 10.1039/D0SC00182A

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Dynamic contrast with reversibly photoswitchable fluorescent labels for imaging living cells

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Dynamic contrast with reversibly photoswitchable fluorescent labels for imaging living cells

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