Issue 67, 2024

Generating cysteine-trypsin cleavage sites with 2-chloroacetamidine capping

Abstract

An electrophilic arginine mimetic, 2-chloroacetamidine (CAM), was deployed to enable trypsin-mediated proteolysis at cysteine residues and to enhance mass spectrometry-based proteomic detection of cysteine-containing peptides. Illustrating the value of the CAM-capping strategy, proteogenomic analysis using a two-stage false discovery rate (FDR) search revealed >50% enhanced coverage of missense variants, when compared to established workflows.

Graphical abstract: Generating cysteine-trypsin cleavage sites with 2-chloroacetamidine capping

Supplementary files

Article information

Article type
Communication
Submitted
10 Apr 2024
Accepted
23 Jul 2024
First published
24 Jul 2024

Chem. Commun., 2024,60, 8856-8859

Generating cysteine-trypsin cleavage sites with 2-chloroacetamidine capping

S. Ofori, H. S. Desai, F. Shikwana, L. M. Boatner, E. R. Dominguez III, J. O. Castellón and K. M. Backus, Chem. Commun., 2024, 60, 8856 DOI: 10.1039/D4CC01583E

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