Issue 31, 2024

Specific interaction between the DSPHTELP peptide and various functional groups

Abstract

M13 bacteriophages serve as a versatile foundation for nanobiotechnology due to their unique biological and chemical properties. The polypeptides that comprise their coat proteins, specifically pVIII, can be precisely tailored through genetic engineering. This enables the customized integration of various functional elements through specific interactions, leading to the development of innovative hybrid materials for applications such as energy storage, biosensing, and catalysis. Notably, a certain genetically engineered M13 bacteriophage variant, referred to as DSPH, features a pVIII with a repeating DSPHTELP peptide sequence. This sequence facilitates specific adhesion to single-walled carbon nanotubes (SWCNTs), primarily through π–π and hydrophobic interactions, though the exact mechanism remains unconfirmed. In this study, we synthesized the DSPHTELP peptide (an 8-mer peptide) and analyzed its interaction forces with different functional groups across various pH levels using surface forces apparatus (SFA). Our findings indicate that the 8-mer peptide binds most strongly to CH3 groups (Wad = 13.74 ± 1.04 mJ m−2 at pH 3.0), suggesting that hydrophobic interactions are indeed the predominant mechanism. These insights offer both quantitative and qualitative understanding of the molecular interaction mechanisms of the 8-mer peptide and clarify the basis of its specific interaction with SWCNTs through the DSPHTELP M13 bacteriophage.

Graphical abstract: Specific interaction between the DSPHTELP peptide and various functional groups

Supplementary files

Article information

Article type
Paper
Submitted
27 Apr 2024
Accepted
12 Jul 2024
First published
15 Jul 2024
This article is Open Access
Creative Commons BY-NC license

Phys. Chem. Chem. Phys., 2024,26, 20760-20769

Specific interaction between the DSPHTELP peptide and various functional groups

H. Kwon, S. Jin, J. Ko, J. Ryu, J. Ryu and D. W. Lee, Phys. Chem. Chem. Phys., 2024, 26, 20760 DOI: 10.1039/D4CP01739K

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