An artificial protein-probe hybrid as a responsive probe for ratiometric detection and imaging of hydrogen peroxide in cells

Abstract

An artificial protein-probe hybrid, Cm–Np-B@BSA, was prepared via host–guest interactions between hydrogen peroxide (H₂O₂)-responsive Cm–Np-B molecule and bovine serum albumin (BSA). The Cm–Np-B@BSA probe exhibited high sensitivity and selectivity towards H₂O₂ under physiological conditions and had excellent biocompatibility, allowing for sensitive ratiometric detection and imaging of endogenous H₂O₂ in live cells.