Issue 25, 2022
From the journal:

RSC Advances

A novel G·G·T non-conventional intramolecular triplex formed by the double repeat sequence of Chlamydomonas telomeric DNA†

Aparna Bansal,ab   Priyanka Phogata  and  Shrikant Kukreti ORCID logo *a  

* Corresponding authors

a Nucleic Acids Research Lab, Department of Chemistry, University of Delhi (North Campus), Delhi, India
E-mail: skukreti@chemistry.du.ac.in, shrikant.kukreti6@gmail.com

b Department of Chemistry, Hansraj College, University of Delhi, (North Campus), Delhi, India

Abstract

Competition among DNA non-canonical structures has been widely studied in repetitive DNA sequences. The factors affecting DNA structural polymorphism have always been an important area of research. The Chlamydomonas reinhardtii telomere (TTTTAGGG)n is found to be an exception to the general idea of forming a folded G-quadruplex by a few repeats in any telomeric sequence. Herein, using gel electrophoresis, UV thermal melting, UV thermal difference spectra (TDS), circular dichroism, and fluorescence and NMR spectroscopy. We demonstrated that the double repeat of the C. reinhardtii telomere (TTTTAGGG)2 (Chlm2) adopts an intramolecular non-conventional triplex structure in Na+ solution. We report that the co-existence of reverse Hoogsteen (G·G) and Wobble base pairing (G·T) stabilizes the triplex structure. To the best of our knowledge, such a non-conventional triplex structure formed by any telomeric sequence has not been reported to date.

Graphical abstract: A novel G·G·T non-conventional intramolecular triplex formed by the double repeat sequence of Chlamydomonas telomeric DNA

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DOI
https://doi.org/10.1039/D2RA00861K
Article type
Paper
Submitted
09 Feb 2022
Accepted
08 Apr 2022
First published
26 May 2022
This article is Open Access
Creative Commons BY-NC license

RSC Adv., 2022,12, 15918-15924

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A novel G·G·T non-conventional intramolecular triplex formed by the double repeat sequence of Chlamydomonas telomeric DNA

A. Bansal, P. Phogat and S. Kukreti, RSC Adv., 2022, 12, 15918 DOI: 10.1039/D2RA00861K

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