Volume 240, 2022
From the journal:

Faraday Discussions

Maintaining the momentum in cryoEM for biological discovery†

Yehuda Halfon,a   Louie Aspinall,a   Joshua White,a   Isobel Jackson Hirst,b   Yiheng Wang,b   Michele C. Darrow,cd   Stephen P. Muench ORCID logo b  and  Rebecca F. Thompson ORCID logo *a  

* Corresponding authors

a School of Molecular and Cellular Biology, Faculty of Biological Sciences & Astbury Centre for Structural and Molecular Biology, University of Leeds, Leeds, UK
E-mail: r.f.thompson@leeds.ac.uk

b School of Biomedical Sciences, Faculty of Biological Sciences & Astbury Centre for Structural and Molecular Biology, University of Leeds, Leeds, UK

c The Rosalind Franklin Institute, Harwell Campus, Didcot, UK

d SPT Labtech Ltd, Melbourn Science Park, Melbourn, UK

Abstract

Cryo-electron microscopy (cryoEM) has been transformed over the last decade, with continual new hardware and software tools coming online, pushing the boundaries of what is possible and the nature and complexity of projects that can be undertaken. Here we discuss some recent trends and new tools which are creating opportunities to make more effective use of the resources available within facilities (both staff and equipment). We present approaches for the stratification of projects based on risk and known information about the projects, and the impacts this might have on the allocation of microscope time. We show that allocating different resources (microscope time) based on this information can lead to a significant increase in ‘successful’ use of the microscope, and reduce lead time by enabling projects to ‘fail faster’. This model results in more efficient and sustainable cryoEM facility operation.

Graphical abstract: Maintaining the momentum in cryoEM for biological discovery

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Article information

DOI
https://doi.org/10.1039/D2FD00129B
Article type
Paper
Submitted
17 Jun 2022
Accepted
11 Jul 2022
First published
15 Jul 2022
This article is Open Access
Creative Commons BY-NC license

Faraday Discuss., 2022,240, 18-32

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Maintaining the momentum in cryoEM for biological discovery

Y. Halfon, L. Aspinall, J. White, I. Jackson Hirst, Y. Wang, M. C. Darrow, S. P. Muench and R. F. Thompson, Faraday Discuss., 2022, 240, 18 DOI: 10.1039/D2FD00129B

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