Issue 64, 2020

Antimicrobial peptide-modified silver nanoparticles for enhancing the antibacterial efficacy

Abstract

Antibiotic-resistant bacteria are becoming a serious threat to public health worldwide. To address this problem, we have developed multifunctional peptide (MFP)-coated silver nanoparticles (MFP@AgNPs) for antibacterial studies. MFPs, which can physically adsorb to AgNPs via electrostatic interactions are comprised of a matrix metalloproteinase (MMP) cleavable sequence (PVGLIG), an antimicrobial peptide (tachyplesin-1), and a target peptide (PGP-PEG). The resulting MFP@AgNPs were characterized by various technologies, including UV-vis spectrophotometry, zeta potential analyzer, circular dichroism (CD) spectroscopy, attenuated total reflection-Fourier-transform infrared spectroscopy (ATR-FTIR), and transmission electron microscopy (TEM). The MIC and MBC were investigated against both Gram-positive bacteria and Gram-negative bacteria. The antibacterial activity in vivo was evaluated on MDR-AB (multidrug-resistant Acinetobacter baumannii) infected mice. We found that MFP@AgNPs exhibited antibacterial activity against both Gram-positive bacteria and Gram-negative bacteria. Compared to bare AgNPs, MFP@AgNPs-1 killed MDR-AB faster and more efficiently. SEM images showed that MFP@AgNPs-1 induced cell disruption via cell membrane damage. In vivo studies further confirmed the enhanced antibacterial activity against MDR-AB infections. The developed MFP@AgNPs-1 reduced the cytotoxicity of AgNPs and enhanced the antibacterial activity against MDR-AB in vitro and in vivo, providing a possible solution against multidrug-resistant bacterial infections.

Graphical abstract: Antimicrobial peptide-modified silver nanoparticles for enhancing the antibacterial efficacy

Associated articles

Supplementary files

Article information

Article type
Paper
Submitted
28 Jun 2020
Accepted
05 Oct 2020
First published
22 Oct 2020
This article is Open Access
Creative Commons BY-NC license

RSC Adv., 2020,10, 38746-38754

Antimicrobial peptide-modified silver nanoparticles for enhancing the antibacterial efficacy

W. Li, Y. Li, P. Sun, N. Zhang, Y. Zhao, S. Qin and Y. Zhao, RSC Adv., 2020, 10, 38746 DOI: 10.1039/D0RA05640E

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