Issue 46, 2019
From the journal:

RSC Advances

A highly selective fluorescent probe for human NAD(P)H:quinone oxidoreductase 1 (hNQO1) detection and imaging in living tumor cells†

Ya Zhu,‡a   Jialing Han,‡a   Qian Zhang,a   Zhou Zhao,a   Jin Wang,a   Xiaowei Xu, ORCID logo *a   Haiping Hao*a  and  Jun Zhang*b  

* Corresponding authors

a School of Pharmacy, China Pharmaceutical University, Nanjing, China
E-mail: xw@cpu.edu.cn, haipinghao@cpu.edu.cn

b School of Pharmacy, Nanjing Medical University, Nanjing, China
E-mail: zhangjun@njmu.edu.cn

Abstract

Human NAD(P)H:quinone oxidoreductase (hNQO1) can be used as a biomarker for the early diagnosis of cancer. In this article, a novel fluorescent probe 1 for detecting hNQO1 was designed and synthesized by using 2-dicyanomethylene-3-cyano-4,5,5-trimethyl-2,5-dihydrofuran (TCF) derivative (TCF-OH) as a fluorophore and quinone propionic acid (QPA) as a recognition group. The probe, which has high selectivity for hNQO1 and a Stokes shift of about 117 nm, shows a linear relationship with hNQO1 concentrations in the range of 0.25–3 μg ml−1 with the color changes from yellow to red and was successfully applied to intracellular hNQO1 imaging.

Graphical abstract: A highly selective fluorescent probe for human NAD(P)H:quinone oxidoreductase 1 (hNQO1) detection and imaging in living tumor cells

About
Cited by
Related
Download options Please wait...

Associated articles

Supplementary files

Article information

DOI
https://doi.org/10.1039/C9RA05650E
Article type
Paper
Submitted
22 Jul 2019
Accepted
20 Aug 2019
First published
27 Aug 2019
This article is Open Access
Creative Commons BY-NC license

RSC Adv., 2019,9, 26729-26733

Permissions

Request permissions

A highly selective fluorescent probe for human NAD(P)H:quinone oxidoreductase 1 (hNQO1) detection and imaging in living tumor cells

Y. Zhu, J. Han, Q. Zhang, Z. Zhao, J. Wang, X. Xu, H. Hao and J. Zhang, RSC Adv., 2019, 9, 26729 DOI: 10.1039/C9RA05650E

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. You can use material from this article in other publications, without requesting further permission from the RSC, provided that the correct acknowledgement is given and it is not used for commercial purposes.

To request permission to reproduce material from this article in a commercial publication, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party commercial publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements