Preparation of a novel injectable in situ-gelling nanoparticle with applications in controlled protein release and cancer cell entrapment

Min Kyung Khang; Jun Zhou; Yihui Huang; Amirhossein Hakamivala; Liping Tang

doi:10.1039/C8RA06589F

Preparation of a novel injectable in situ-gelling nanoparticle with applications in controlled protein release and cancer cell entrapment

Min Kyung Khang,

^ab Jun Zhou,^b Yihui Huang,^b Amirhossein Hakamivala^b and Liping Tang

*^bc

Author affiliations

* Corresponding authors

^a Chemistry and Biochemistry Department, University of Texas at Arlington, Arlington, Texas, USA

^b Bioengineering Department, University of Texas at Arlington, P. O. Box 19138, Arlington, Texas 76019-0138, USA
E-mail: ltang@uta.edu

^c Department of Biomedical Science and Environmental Biology, Kaohsiung Medical University, Kaohsiung 807, Taiwan

Abstract

Temperature sensitive injectable hydrogels have been used as drug/protein carriers for a variety of pharmaceutical applications. Oligo(ethylene glycol) methacrylate (OEGMA) monomers with varying ethylene oxide chain lengths have been used for the synthesis of in situ forming hydrogel. In this study, a new series of thermally induced gelling hydrogel nanoparticles (PMOA hydrogel nanoparticles) was developed by copolymerization with di(ethylene glycol) methyl ether methacrylate (MEO₂MA), poly(ethylene glycol) methyl ether methacrylate (300 g mol⁻¹, OEGMA₃₀₀), and acrylic acid (AAc). The effects of acrylic acid content on the physical, chemical, and biological properties of the nanoparticle-based hydrogels were investigated. Due to its high electrostatic properties, addition of AAc increases LCST as well as gelation temperature. Further, using Cy5-labelled bovine serum albumin and erythropoietin (Epo) as model drugs, studies have shown that the thermogelling hydrogels have the ability to tune the release rate of these proteins in vitro. Finally, the ability of Epo releasing hydrogels to recruit prostate cancer cells was assessed in vivo. Overall, our results support that this new series of thermally induced gelling systems can be used as protein control releasing vehicles and cancer cell traps.

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DOI: https://doi.org/10.1039/C8RA06589F
Article type: Paper
Submitted: 05 Aug 2018
Accepted: 02 Oct 2018
First published: 09 Oct 2018
This article is Open Access

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RSC Adv., 2018,8, 34625-34633

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Preparation of a novel injectable in situ-gelling nanoparticle with applications in controlled protein release and cancer cell entrapment

M. K. Khang, J. Zhou, Y. Huang, A. Hakamivala and L. Tang, RSC Adv., 2018, 8, 34625 DOI: 10.1039/C8RA06589F

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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Preparation of a novel injectable in situ-gelling nanoparticle with applications in controlled protein release and cancer cell entrapment

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Preparation of a novel injectable in situ-gelling nanoparticle with applications in controlled protein release and cancer cell entrapment

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