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The first systematic investigation of the effect of high mannose, hybrid, and bi- and tri-antennary complex type glycans on the effector functions of antibodies was achieved by the discovery of novel Endo-S2 mutants generated by site-directed mutagenesis as glycosynthases with broad substrate specificity.

Graphical abstract: Development of glycosynthases with broad glycan specificity for the efficient glyco-remodeling of antibodies

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