Biological fluorination from the sea: discovery of a SAM-dependent nucleophilic fluorinating enzyme from the marine-derived bacterium Streptomyces xinghaiensis NRRL B24674

Long Ma; Yufeng Li; Lingpei Meng; Hai Deng; Yuyin Li; Qiang Zhang; Aipo Diao

doi:10.1039/C6RA00100A

Biological fluorination from the sea: discovery of a SAM-dependent nucleophilic fluorinating enzyme from the marine-derived bacterium Streptomyces xinghaiensis NRRL B24674†

Long Ma,*^abc Yufeng Li,^ab Lingpei Meng,^ab Hai Deng,^d Yuyin Li,^ab Qiang Zhang^e and Aipo Diao^ab

* Corresponding authors

^a Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, School of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China
E-mail: malong@tust.edu.cn
Fax: +86 22 60602298
Tel: +86 22 60602948

^b Tianjin Key Laboratory of Industry Microbiology, School of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China
Tel: +86 133 446 7254

^c Biomolecular Sciences Research Complex, EaStCHEM School of Chemistry, University of St Andrews, Fife KY16 9ST, UK
E-mail: lm228@st.andrews.ac.uk

^d Marine Biodiscovery Centre, Department of Chemistry, University of Aberdeen, Meston Walk, Aberdeen AB24 3UE, UK

^e Tianjin 3rd Center Hospital, Tianjin 300170, China

Abstract

We have discovered and characterised a fluorinating enzyme from marine-derived Streptomyces xinghaiensis. It is able to install a fluorine atom into S-adenosyl-L-methionine (SAM) to form 5′-fluorodeoxy adenosine. To our knowledge, this is the first ever fluorinase unveiled from a marine source. It is ‘the most efficient’ fluorinase by far and, impressively, highly robust. Therefore it promises to be useful in different cases such as bio-transformation and synthetic biology.

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DOI: https://doi.org/10.1039/C6RA00100A
Article type: Communication
Submitted: 03 Jan 2016
Accepted: 27 Feb 2016
First published: 02 Mar 2016
This article is Open Access

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RSC Adv., 2016,6, 27047-27051

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Biological fluorination from the sea: discovery of a SAM-dependent nucleophilic fluorinating enzyme from the marine-derived bacterium Streptomyces xinghaiensis NRRL B24674

L. Ma, Y. Li, L. Meng, H. Deng, Y. Li, Q. Zhang and A. Diao, RSC Adv., 2016, 6, 27047 DOI: 10.1039/C6RA00100A

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Biological fluorination from the sea: discovery of a SAM-dependent nucleophilic fluorinating enzyme from the marine-derived bacterium Streptomyces xinghaiensis NRRL B24674†

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Biological fluorination from the sea: discovery of a SAM-dependent nucleophilic fluorinating enzyme from the marine-derived bacterium Streptomyces xinghaiensis NRRL B24674

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