Microfluidic flow cytometry for blood-based biomarker analysis

Yuxin Zhang, Ying Zhao, Tim Cole, Jiahao Zheng, Bayinqiaoge, Jinhong Guo and Shi-Yang Tang

Analyst, 2022, 147, 2895
DOI: 10.1039/D2AN00283C

Recent advances in single cell manipulation and biochemical analysis on microfluidics

Dan Gao, Feng Jin, Min Zhou and Yuyang Jiang

Analyst, 2019, 144, 766
DOI: 10.1039/C8AN01186A

CITEMO XMBD : A flexible single-cell multimodal omics analysis framework to reveal the heterogeneity of immune cells

Huan Hu, Ruiqi Liu, Chunlin Zhao, Yuer Lu, Yichun Xiong, Lingling Chen, Jun Jin, Yunlong Ma, Jianzhong Su, Zhengquan Yu, Feng Cheng, Fangfu Ye, Liyu Liu, Qi Zhao and Jianwei Shuai

RNA Biology, 2022, 19, 290
DOI: 10.1080/15476286.2022.2027151

Integrated Cardiomyocyte-Based Biosensing Platform for Electroporation-Triggered Intracellular Recording in Parallel with Delivery Efficiency Evaluation

Jiaru Fang, Yuxiang Pan, Jiarong Xu, Dongxin Xu, Hongbo Li, Chuan Liu and Ning Hu

Nano Lett., 2023, 23, 4049
DOI: 10.1021/acs.nanolett.3c00917

Cross Channel Thread-Based Microfluidic Device for Separation of Food Dyes

Chunxiu Xu, Danli Jiang, Jiayi Lin and Longfei Cai

J. Chem. Educ., 2018, 95, 1000
DOI: 10.1021/acs.jchemed.7b00784

Quantification of Low Copy Number Proteins in Single Cells

Meng Shi, Xuhui Geng, Chengye Wang and Yafeng Guan

Anal. Chem., 2019, 91, 11493
DOI: 10.1021/acs.analchem.9b02989

Absolute Quantification of Protein Copy Number in Single Cells With Immunofluorescence Microscopy Calibrated Using Single-Molecule Microarrays

Stelios Chatzimichail, Pashiini Supramaniam and Ali Salehi-Reyhani

Anal. Chem., 2021, 93, 6656
DOI: 10.1021/acs.analchem.0c05177

Cross-Correlative Single-Cell Analysis Reveals Biological Mechanisms of Nanoparticle Radiosensitization

Tyron Turnbull, Michael Douglass, Nathan H. Williamson, Douglas Howard, Richa Bhardwaj, Mark Lawrence, David J. Paterson, Eva Bezak, Benjamin Thierry and Ivan M. Kempson

ACS Nano, 2019, 13, 5077
DOI: 10.1021/acsnano.8b07982

Microfluidics towards single cell resolution protein analysis

Peng Chen, Dongjuan Chen, Shunji Li, Xiaowen Ou and Bi-Feng Liu

TrAC Trends in Analytical Chemistry, 2019, 117, 2
DOI: 10.1016/j.trac.2019.06.022

Single-cell HER2 quantification via instant signal amplification in microdroplets

Xiaoxian Liu, Yifan Zhu, Caoxin Li, Yanyun Fang, Jinna Chen, Fei Xu, Yanqing Lu, Perry Ping Shum, Ying Liu and Guanghui Wang

Analytica Chimica Acta, 2023, 1251, 340976
DOI: 10.1016/j.aca.2023.340976

A Miniature Modular Fluorescence Flow Cytometry System

Shaoqi Huang, Jiale Li, Li Wei, Lulu Zheng, Zheng Shi, Shiwei Guo, Bo Dai, Dawei Zhang and Songlin Zhuang

Biosensors, 2024, 14, 395
DOI: 10.3390/bios14080395

Absolute Copy Numbers of β-Actin Proteins Collected from 10,000 Single Cells

Beiyuan Fan, Xiufeng Li, Lixing Liu, Deyong Chen, Shanshan Cao, Dong Men, Junbo Wang and Jian Chen

Micromachines, 2018, 9, 254
DOI: 10.3390/mi9050254

Development of microfluidic platform capable of high-throughput absolute quantification of single-cell multiple intracellular proteins from tumor cell lines and patient tumor samples

Lixing Liu, Hongyu Yang, Dong Men, Meng Wang, Xiaolei Gao, Ting Zhang, Deyong Chen, Chunlai Xue, Yixiang Wang, Junbo Wang and Jian Chen

Biosensors and Bioelectronics, 2020, 155, 112097
DOI: 10.1016/j.bios.2020.112097

Single Cell Omics: From Assay Design to Biomedical Application

Wenyi Chen, Shumin Li, Anuja Shreeram Kulkarni, Lin Huang, Jing Cao, Kun Qian and Jingjing Wan

Biotechnology Journal, 2020, 15
DOI: 10.1002/biot.201900262

A novel microfluidic flow-cytometry for counting numbers of single-cell β-actins

Lixing Liu, Beiyuan Fan, Hongyu Yang, Deyong Chen, Shuang Zhang, Junbo Wang and Jian Chen

Nanotechnology and Precision Engineering, 2020, 3, 156
DOI: 10.1016/j.npe.2020.06.001

A droplet-based microfluidic flow cytometry enabling absolute quantification of single-cell proteins leveraging constriction channel

Hongyu Yang, Yuanchen Wei, Beiyuan Fan, Lixing Liu, Ting Zhang, Deyong Chen, Junbo Wang and Jian Chen

Microfluid Nanofluid, 2021, 25
DOI: 10.1007/s10404-021-02427-w

Single-Cell Omics Analyses Enabled by Microchip Technologies

Yanxiang Deng, Amanda Finck and Rong Fan

Annu. Rev. Biomed. Eng., 2019, 21, 365
DOI: 10.1146/annurev-bioeng-060418-052538

Microchannel Chip Based on Fluid Dynamics Control for Cell Analysis and Manipulation

Jiao Hua, Zhiwei Fan, Chong Ji, Dongyang Miao, Chaobo Huang and Ranhua Xiong

Adv Funct Materials, 2026, 36
DOI: 10.1002/adfm.202514148

Intercellular transfer of proteins and mitochondria - the contribution of proteomics

Ana Laura Guzmán-Ortiz, Erandi Pérez-Figueroa, Ricardo Valle-Ríos, Karen Melissa Martínez-González and Héctor Quezada

Journal of Proteomics, 2025, 320, 105491
DOI: 10.1016/j.jprot.2025.105491

Recent advances in single-cell ultra-trace analysis

Xing Wei, Yi Lu, Xuan Zhang, Ming-Li Chen and Jian-Hua Wang

TrAC Trends in Analytical Chemistry, 2020, 127, 115886
DOI: 10.1016/j.trac.2020.115886

Review and perspectives on microfluidic flow cytometers

Ruey-Jen Yang, Lung-Ming Fu and Hui-Hsiung Hou

Sensors and Actuators B: Chemical, 2018, 266, 26
DOI: 10.1016/j.snb.2018.03.091

Microfluidic Quantitative Flow Cytometer With Light Modulation

Ting Zhang, Lixing Liu, Hongyu Yang, Yixiang Wang, Deyong Chen, Junbo Wang and Jian Chen

IEEE Sensors J., 2022, 22, 3009
DOI: 10.1109/JSEN.2021.3140110

Advances of 3D Cell Co-Culture Technology Based on Microfluidic Chips

Can Li, Wei He, Yihua Song, Xia Zhang, Jianfei Sun and Zuojian Zhou

Biosensors, 2024, 14, 336
DOI: 10.3390/bios14070336

High throughput cell stiffness measurement via multiplexed impedance sensors

Norh Asmare, A K M Arifuzzman, Ningquan Wang, Mert Boya, Ruxiu Liu and A. Fatih Sarioglu

Biosensors and Bioelectronics, 2025, 273, 117158
DOI: 10.1016/j.bios.2025.117158

Pulsation Reduction Using Dual Sidewall-Driven Micropumps

Takuto Atsumi, Toshio Takayama and Makoto Kaneko

Micromachines, 2022, 14, 19
DOI: 10.3390/mi14010019

Protein and Proteome Measurements with Microfluidic Chips

Iulia M. Lazar, Nicholas S. Gulakowski and Alexandru C. Lazar

Anal. Chem., 2020, 92, 169
DOI: 10.1021/acs.analchem.9b04711

Quantitative flow cytometry leveraging droplet‐based constriction microchannels with high reliability and high sensitivity

Guang Yang, Hongyu Yang, Ting Zhang, Chiyuan Gao, Deyong Chen, Junbo Wang and Jian Chen

Cytometry Pt A, 2023, 103, 429
DOI: 10.1002/cyto.a.24705

A Microfluidic Fluorescent Flow Cytometry Capable of Quantifying Cell Sizes and Numbers of Specific Cytosolic Proteins

Xiufeng Li, Beiyuan Fan, Lixing Liu, Deyong Chen, Shanshan Cao, Dong Men, Junbo Wang and Jian Chen

Sci Rep, 2018, 8
DOI: 10.1038/s41598-018-32333-1

Development of constrictional microchannels and the recurrent neural network in single-cell protein analysis

Ting Zhang, Xiao Chen, Deyong Chen, Junbo Wang and Jian Chen

Front. Bioeng. Biotechnol., 2023, 11
DOI: 10.3389/fbioe.2023.1195940

Development of a Microfluidic Flow Cytometer with a Uniform Optical Field (Uni-μFCM) Enabling Quantitative Analysis of Single-Cell Proteins and Its Applications in Leukemia Gating, Tumor Classification, and Hierarchy of Cancer Stem Cells

Chiyuan Gao, Ting Zhang, Yuanchen Wei, Qinghua Liu, Liangliang Ma, Mengge Gao, Xiaosu Zhao, Yixiang Wang, Deyong Chen, Lichao Sun, Junbo Wang and Jian Chen

ACS Sens., 2023, 8, 3498
DOI: 10.1021/acssensors.3c01060

Microfluidic Analyzer Enabling Quantitative Measurements of Specific Intracellular Proteins at the Single-Cell Level

Lixing Liu, Beiyuan Fan, Diancan Wang, Xiufeng Li, Yeqing Song, Ting Zhang, Deyong Chen, Yixiang Wang, Junbo Wang and Jian Chen

Micromachines, 2018, 9, 588
DOI: 10.3390/mi9110588

Development of a bubble-based single cell picking system

Ronghang Wang, Bingxin Liu, Jiahao Gong, Jinlu Zhang, Meng Gao, Lunjia Zhang, Xuelin Wang, Sen Chen, Jie Hong and Lin Gui

J. Micromech. Microeng., 2022, 32, 035006
DOI: 10.1088/1361-6439/ac4c96

Phage-Based Profiling of Rare Single Cells Using Nanoparticle-Directed Capture

Yuan Ma, Kangfu Chen, Fan Xia, Randy Atwal, Hansen Wang, Sharif U. Ahmed, Lia Cardarelli, Irene Lui, Bill Duong, Zongjie Wang, James A. Wells, Sachdev S. Sidhu and Shana O. Kelley

ACS Nano, 2021, 15, 19202
DOI: 10.1021/acsnano.1c03935

Advances of Single-Cell Protein Analysis

Lixing Liu, Deyong Chen, Junbo Wang and Jian Chen

Cells, 2020, 9, 1271
DOI: 10.3390/cells9051271

A Simple and Low-cost Preliminary Quantification of Target Membrane Protein in Single Cells

Jiamin Li, Meng Shi, Xuhui Geng and Yafeng Guan

J Fluoresc, 2023, 35, 63
DOI: 10.1007/s10895-023-03496-6

Flow induced particle separation and collection through linear array pillar microfluidics device

Prerna Balyan, Deepika Saini, Supriyo Das, Dhirendra Kumar and Ajay Agarwal

Biomicrofluidics, 2020, 14
DOI: 10.1063/1.5143656

Cellular Analysis Using Microfluidics

Jay Sibbitts, Kathleen A. Sellens, Shu Jia, Scott A. Klasner and Christopher T. Culbertson

Anal. Chem., 2018, 90, 65
DOI: 10.1021/acs.analchem.7b04519

Development of droplet microfluidics capable of quantitative estimation of single-cell multiplex proteins

Hongyu Yang, Guang Yang, Ting Zhang, Deyong Chen, Junbo Wang and Jian Chen

J. Micromech. Microeng., 2022, 32, 024002
DOI: 10.1088/1361-6439/ac4008

Demands and technical developments of clinical flow cytometry with emphasis in quantitative, spectral, and imaging capabilities

Ting Zhang, Mengge Gao, Xiao Chen, Chiyuan Gao, Shilun Feng, Deyong Chen, Junbo Wang, Xiaosu Zhao and Jian Chen

Nanotechnology and Precision Engineering, 2022, 5
DOI: 10.1063/10.0015301