Issue 8, 2014

High throughput MS techniques for caviar lipidomics

Abstract

The lipid profile of Sturgeon roe (caviar) was monitored by matrix assisted laser desorption/ionization mass spectrometry (MALDI(+)-MS), thermal imprinting easy ambient sonic-spray ionization mass spectrometry (TI-EASI(+)-MS) and electrospray mass spectrometry (ESI(+)-MS). Freshly salted and commercially salted pasteurized caviar samples of Atlantic sturgeon (Acipenser sturio) were stored either at +4 °C or at room temperature for 4 weeks. The different types of chemical information achieved by these MS techniques were compared: MALDI(+)-MS detects mainly phospholipids (PL) whereas TI-EASI(+)-MS allows monitoring of both triacylglycerols (TAG) and PL. ESI(+) coupled to Fourier transform ion cyclotron resonance high resolution mass spectrometer (FT-ICR-MS) and MS/MS experiments were used to fully characterize the detected lipids, ensure the absence of oxidation products in the degradation process and confirm the high efficiency of the thermal imprinting extraction. TI-EASI(+)-MS, via a more comprehensive profiling and easier operation, has therefore been demonstrated to provide caviar lipidomic profiles and discriminate its changes as a function of storage time and temperature. The data have also confirmed that hydrolysis is the main process of lipid degradation in caviar.

Graphical abstract: High throughput MS techniques for caviar lipidomics

Article information

Article type
Paper
Submitted
04 Nov 2013
Accepted
28 Dez 2013
First published
06 Jan 2014

Anal. Methods, 2014,6, 2436-2443

High throughput MS techniques for caviar lipidomics

A. M. Porcari, G. D. Fernandes, K. R. A. Belaz, N. V. Schwab, V. G. Santos, R. M. Alberici, V. A. Gromova, M. N. Eberlin, A. T. Lebedev and A. Tata, Anal. Methods, 2014, 6, 2436 DOI: 10.1039/C3AY41954A

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