Rh single-atom nanozymes for efficient ascorbic acid oxidation and detection

Abstract

The management of ascorbic acid (AA) in biological fluids is of significant importance for body functions and human health, yet challenging due to the lack of high-performance sensing catalysts. Herein, we report the design of Rh single-atom nanozymes (Rh SAzymes) by mimicking the active sites of ascorbate peroxidase toward efficient electrocatalytic oxidation and detection of AA. Benefiting from the enzyme-mimicking single-atom coordination, the Rh SAzyme exhibits an unprecedented electrocatalytic activity for AA oxidation with an onset potential as low as 0.02 V (vs. Ag/AgCl). Combined with the screen-printing technology, a miniaturized Rh SAzyme biosensor was firstly constructed for tracking dynamic trends of AA in the human subject and detecting AA content in nutritional products. The as-prepared biosensor exhibits excellent detection performances with a wide linear range of 10.0 μM–53.1 mM, a low detection limit of 0.26 μM, and a long stability of 28 days. This work opens a door for the design of artificial single-atom electrocatalysts to mimic natural enzymes and their subsequent application in biosensors.