Issue 5, 2023

Microwell array chip-based single-cell analysis

Abstract

Single-cell profiling is key to uncover the cellular heterogeneity and drives deep understanding of cell fate. In recent years, microfluidics has become an ideal tool for single-cell profiling owing to its benefits of high throughput and automation. Among various microfluidic platforms, microwell has the advantages of simple operation and easy integration with in situ analysis ability, making it an ideal technique for single-cell studies. Herein, recent advances of single-cell analysis based on microwell array chips are summarized. We first introduce the design and preparation of different microwell chips. Then microwell-based cell capture and lysis strategies are discussed. We finally focus on advanced microwell-based analysis of single-cell proteins, nucleic acids, and metabolites. The challenges and opportunities for the development of microwell-based single-cell analysis are also presented.

Graphical abstract: Microwell array chip-based single-cell analysis

Article information

Article type
Critical Review
Submitted
19 Jul 2022
Accepted
11 Okt 2022
First published
10 Jan 2023

Lab Chip, 2023,23, 1066-1079

Microwell array chip-based single-cell analysis

J. Zhang, J. Xue, N. Luo, F. Chen, B. Chen and Y. Zhao, Lab Chip, 2023, 23, 1066 DOI: 10.1039/D2LC00667G

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