Issue 10, 2023

Molecular dynamics simulation-based trinucleotide and tetranucleotide level structural and energy characterization of the functional units of genomic DNA

Abstract

Genomes of most organisms on earth are written in a universal language of life, made up of four units – adenine (A), thymine (T), guanine (G), and cytosine (C), and understanding the way they are put together has been a great challenge to date. Multiple efforts have been made to annotate this wonderfully engineered string of DNA using different methods but they lack a universal character. In this article, we have investigated the structural and energetic profiles of both prokaryotes and eukaryotes by considering two essential genomic sites, viz., the transcription start sites (TSS) and exon–intron boundaries. We have characterized these sites by mapping the structural and energy features of DNA obtained from molecular dynamics simulations, which considers all possible trinucleotide and tetranucleotide steps. For DNA, these physicochemical properties show distinct signatures at the TSS and intron–exon boundaries. Our results firmly convey the idea that DNA uses the same dialect for prokaryotes and eukaryotes and that it is worth going beyond sequence-level analyses to physicochemical space to determine the functional destiny of DNA sequences.

Graphical abstract: Molecular dynamics simulation-based trinucleotide and tetranucleotide level structural and energy characterization of the functional units of genomic DNA

Supplementary files

Article information

Article type
Paper
Submitted
15 Okt 2022
Accepted
09 Feb 2023
First published
10 Feb 2023

Phys. Chem. Chem. Phys., 2023,25, 7323-7337

Molecular dynamics simulation-based trinucleotide and tetranucleotide level structural and energy characterization of the functional units of genomic DNA

D. Sharma, K. Sharma, A. Mishra, P. Siwach, A. Mittal and B. Jayaram, Phys. Chem. Chem. Phys., 2023, 25, 7323 DOI: 10.1039/D2CP04820E

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