Issue 75, 2022

A hydrogen-driven biocatalytic approach to recycling synthetic analogues of NAD(P)H

Abstract

We demonstrate a recycling system for synthetic nicotinamide cofactor analogues using a soluble hydrogenase with turnover number of >1000 for reduction of the cofactor analogues by H2. Coupling this system to an ene reductase, we show quantitative conversion of N-ethylmaleimide to N-ethylsuccinimide. The biocatalyst system retained >50% activity after 7 h.

Graphical abstract: A hydrogen-driven biocatalytic approach to recycling synthetic analogues of NAD(P)H

Supplementary files

Article information

Article type
Communication
Submitted
01 Mai 2022
Accepted
17 Aug 2022
First published
18 Aug 2022
This article is Open Access
Creative Commons BY license

Chem. Commun., 2022,58, 10540-10543

A hydrogen-driven biocatalytic approach to recycling synthetic analogues of NAD(P)H

H. A. Reeve, J. Nicholson, F. Altaf, T. H. Lonsdale, J. Preissler, L. Lauterbach, O. Lenz, S. Leimkühler, F. Hollmann, C. E. Paul and K. A. Vincent, Chem. Commun., 2022, 58, 10540 DOI: 10.1039/D2CC02411J

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