Issue 16, 2020

Platinum ions mediate the interactions between DNA and carbon quantum dots: diagnosis of MRSA infections

Abstract

In this study, we have developed a rapid and cost-effective method employing platinum ion (Pt4+)-capped fluorescent carbon quantum dots (CQDs) coupled with loop-mediated isothermal amplification (LAMP) to detect dual MRSA genes. We synthesized nitrogen- and chlorine-co-doped fluorescent CQDs (CQDSPDs) from spermidine trihydrochloride via a simple one-step pyrolysis. The CQDSPDs capped with Pt4+ ions through the cooperative coordination of the amine and chlorine groups on the surface of CQDs facilitated the double-stranded DNA (dsDNA)-induced fluorescence quenching of CQDs, and enabled the construction of the CQDSPDs/Pt4+ probe for the detection of as few as 10 copies of the MRSA gene (mecA and femA). The sensitivity and specificity of the CQDSPDs/Pt4+ probe for MRSA detection in clinical specimens (n = 24) were 94% and 86%, respectively. Our results reveal that the CQDSPDs/Pt4+ probe has great potential for the diagnosis of antibiotic-resistant superbugs with high sensitivity, specificity, and agreement.

Graphical abstract: Platinum ions mediate the interactions between DNA and carbon quantum dots: diagnosis of MRSA infections

  • This article is part of the themed collection: Biosensors

Supplementary files

Article information

Article type
Communication
Submitted
04 Nov 2019
Accepted
06 Dez 2019
First published
06 Dez 2019

J. Mater. Chem. B, 2020,8, 3506-3512

Platinum ions mediate the interactions between DNA and carbon quantum dots: diagnosis of MRSA infections

H. Li, J. Mao, C. Lien, C. Wang, J. Lai, R. P. Mandal, H. Chang, L. Chang, D. H. Ma and C. Huang, J. Mater. Chem. B, 2020, 8, 3506 DOI: 10.1039/C9TB02468A

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