Issue 45, 2018

A potentiometric aptasensor for carcinoembryonic antigen (CEA) on graphene oxide nanosheets using catalytic recycling of DNase I with signal amplification

Abstract

A new and label-free potentiometric aptasensing platform was designed for the sensitive detection of carcinoembryonic antigen (CEA) in human serum on a graphene oxide (GO) nanosheet-modified glassy carbon electrode by coupling with target recycling-assisted signal amplification. To construct such a signal-amplification aptasensing system, the nanosheets were initially immobilized on the electrode through physical adsorption, and then CEA aptamers were coated on the nanosheets via π-stacking interaction. Upon target CEA introduction, the analyte reacted with the aptamer to form a complex, thus resulting in the dissociation of the aptamer from the nanosheets. The formed CEA–aptamer complexes were cleaved in the presence of DNase I to release target CEA, thereby triggering the target recycling and catalytic recycling of DNase I. Thanks to the negatively charged oligonucleotide skeleton, the dissociation of the aptamers from the nanosheets could cause a change in the local electrical potential of the modified electrode. Under optimum conditions, the shift in the potential increased with the increment of target CEA concentrations, and exhibited good potential responses within a linear range of 0.01–100 ng mL−1 at a low detection limit of 9.4 pg mL−1. The specificity, precision, reproducibility and stability of the potentiometric aptasensor were acceptable. The accuracy of this method was evaluated for the analysis of human serum specimens, giving well-matched results with those obtained from the commercial human CEA ELISA kit.

Graphical abstract: A potentiometric aptasensor for carcinoembryonic antigen (CEA) on graphene oxide nanosheets using catalytic recycling of DNase I with signal amplification

Article information

Article type
Paper
Submitted
27 Sep 2018
Accepted
28 Okt 2018
First published
29 Okt 2018

Anal. Methods, 2018,10, 5364-5371

A potentiometric aptasensor for carcinoembryonic antigen (CEA) on graphene oxide nanosheets using catalytic recycling of DNase I with signal amplification

Z. Hong, G. Chen, S. Yu, R. Huang and C. Fan, Anal. Methods, 2018, 10, 5364 DOI: 10.1039/C8AY02113A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements