Issue 17, 2015
From the journal:

Analytical Methods

Microfluidic protein crystallisation controlled using spontaneous emulsification†

Mao Fukuyama,*ab   Aoi Akiyama,a   Makoto Harada,a   Tetsuo Okadaa  and  Akihide Hibara*a  

* Corresponding authors

a Tokyo Institute of Technology, Ookayama, Meguro-ku, Tokyo 152-8551, Japan
E-mail: fukuyama.m.aa@m.titech.ac.jp, ahibara@chem.titech.ac.jp

b Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan
E-mail: maofukuyama@kit.ac.jp

Abstract

Microdroplet-based protein crystallisation using spontaneous emulsification is proposed and demonstrated. The dependence of water transport on the concentration of Span 80 was investigated. Crystallisation of the lysozyme was then demonstrated. Based on the results obtained, the dependency of the crystal number in a single microdroplet on the surfactant concentration was discussed.

Graphical abstract: Microfluidic protein crystallisation controlled using spontaneous emulsification

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Article information

DOI
https://doi.org/10.1039/C5AY00578G
Article type
Communication
Submitted
05 Mrz 2015
Accepted
15 Apr 2015
First published
16 Apr 2015
This article is Open Access
Creative Commons BY license

Anal. Methods, 2015,7, 7128-7131

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Microfluidic protein crystallisation controlled using spontaneous emulsification

M. Fukuyama, A. Akiyama, M. Harada, T. Okada and A. Hibara, Anal. Methods, 2015, 7, 7128 DOI: 10.1039/C5AY00578G

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