Issue 17, 2015

Hydrodynamic nonadhesive cell retention in a microfluidic circuit for stressless suspension culture

Abstract

Cell collection based on deterministic lateral displacement (DLD) and cell circulation with a loop channel are two component technologies for stressless cell retention which have been developed with a view to working toward suspension culture in a microfluidic channel. DLD devices with low array angles collect floating S. cerevisiae through an array effectively. The DLD device with an array angle of 2.6° showed an efficiency of 91.7%. Two types of loop channels with a piezoelectrie pump make a stable two-phase laminar flow of a pre-filled fluid and supplied fluid. A loop channel with a connection between an inlet and outlet on the opposite side replaces a filled fluid in the channel with a supplied fluid, which is essential for supplying nutrient rich medium to cells in microfluidic channels as well as retaining cells in a microenvironment without external stresses.

Graphical abstract: Hydrodynamic nonadhesive cell retention in a microfluidic circuit for stressless suspension culture

Supplementary files

Article information

Article type
Paper
Submitted
24 Feb 2015
Accepted
14 Apr 2015
First published
06 Mai 2015

Anal. Methods, 2015,7, 7264-7269

Hydrodynamic nonadhesive cell retention in a microfluidic circuit for stressless suspension culture

T. Naito, N. Kaji, M. Tokeshi, T. Kubo, Y. Baba and K. Otsuka, Anal. Methods, 2015, 7, 7264 DOI: 10.1039/C5AY00485C

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements