Issue 17, 2015

Exploratory analysis of avocado extracts by GC-MS: new insights into the avocado fruit ripening process

Abstract

Herewith the usefulness of a gas chromatography-electron ionization-quadrupole mass spectrometry (GC-EI-Q MS) methodology has been evaluated for the identification of potential ripening or variety markers of the avocado fruit. A selection of 13 avocado varieties at two diverse ripening degrees was made and their derivatized extracts were analyzed by GC-MS. Even though the method was able to identify about 60 metabolites within a single run, the approach used was more exploratory, and data processing and analysis steps were carried out, implying filtering, normalization, principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA). 17 compounds were selected as the most influential to be considered in PCA and clustering analysis by the k-means method. To corroborate further on the metabolic changes associated with ripening, a two class PLS-DA model was built, identifying abscisic acid, linoleic acid, aspartic acid and mannoheptulose as the compounds with the highest influence in the model.

Graphical abstract: Exploratory analysis of avocado extracts by GC-MS: new insights into the avocado fruit ripening process

Article information

Article type
Paper
Submitted
23 Mrz 2015
Accepted
07 Mai 2015
First published
28 Mai 2015

Anal. Methods, 2015,7, 7318-7326

Exploratory analysis of avocado extracts by GC-MS: new insights into the avocado fruit ripening process

E. Hurtado-Fernández, A. Bajoub, J. C. Morales, A. Fernández-Gutiérrez and A. Carrasco-Pancorbo, Anal. Methods, 2015, 7, 7318 DOI: 10.1039/C5AY00767D

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements