Issue 41, 2025
From the journal:

Chemical Communications

A novel CRISPR–Cas12a-based fluorescence anisotropy method with a high signal-to-background ratio for sensitive biosensing†

Yu Qin,‡a   Jia Li Xie,‡a   Kai Mao,a   Yuan Fang Li, ORCID logo a   Cheng Zhi Huang, ORCID logo b   Hong Yan Zou ORCID logo *b  and  Shu Jun Zhen ORCID logo *a  

* Corresponding authors

a College of Chemistry and Chemical Engineering, Southwest University, Chongqing, P. R. China
E-mail: zsj@swu.edu.cn

b Key Laboratory of Biomedical Analytics, Chongqing Science and Technology Bureau, College of Pharmaceutical Sciences, Southwest University, Chongqing, P. R. China
E-mail: zhy2013@swu.edu.cn

Abstract

Here, a CRISPR–Cas12a system with high trans-cleavage ability integrating a DNA nanochain formed by DNA tetrahedrons with a large molecular mass was employed to enhance the signal-to-background ratio of the fluorescence anisotropy method for achieving sensitive detection of hepatitis B virus DNA.

Graphical abstract: A novel CRISPR–Cas12a-based fluorescence anisotropy method with a high signal-to-background ratio for sensitive biosensing

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Article information

DOI
https://doi.org/10.1039/D5CC01565K
Article type
Communication
Submitted
19 Mär 2025
Accepted
11 Apr 2025
First published
17 Apr 2025

Chem. Commun., 2025,61, 7458-7461

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A novel CRISPR–Cas12a-based fluorescence anisotropy method with a high signal-to-background ratio for sensitive biosensing

Y. Qin, J. L. Xie, K. Mao, Y. F. Li, C. Z. Huang, H. Y. Zou and S. J. Zhen, Chem. Commun., 2025, 61, 7458 DOI: 10.1039/D5CC01565K

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