Issue 65, 2022

Selectivity and efficiency in the ligation of the pyrene:abasic base pair by T4 and PBCV-1 DNA ligases

Abstract

Remarkable selectivity was observed in the ligation of 5′-phosphate 1-pyrene nucleotide terminated strands across from an abasic lesion in a DNA-templated ligation reaction by two different ligases suggesting that pyrene-terminated strands could be used in abasic site detection. Increasing ATP concentration was critical to enhancing the selectivity for this base pair with T4 DNA ligase.

Graphical abstract: Selectivity and efficiency in the ligation of the pyrene:abasic base pair by T4 and PBCV-1 DNA ligases

Supplementary files

Article information

Article type
Communication
Submitted
31 Mai 2022
Accepted
13 Jul 2022
First published
18 Jul 2022

Chem. Commun., 2022,58, 9072-9075

Selectivity and efficiency in the ligation of the pyrene:abasic base pair by T4 and PBCV-1 DNA ligases

H. Park and J. M. Gibbs, Chem. Commun., 2022, 58, 9072 DOI: 10.1039/D2CC03079A

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