Issue 12, 2011

Inductively coupled plasma mass spectrometry for determination of total urinary protein with CdTequantum dots label

Abstract

In the present work, a novel methodology for sensitive quantification of human total urinary protein by using CdTe quantum dots label and inductively coupled plasma mass spectrometry (ICP-MS) was developed. It combined the intrinsically high sensitivity of elemental mass spectrometric analysis with the remarkable quantity of metallic ions from the nanoparticles label. Through the direct conjugation of CdTe quantum dots and proteins, the ICP-MS signal intensity of cadmium is proportional to the concentration of proteins. The optimization of bio-conjugation parameters was investigated and discussed in detail. The proposed method featured a low detection limit (0.008 μg mL−1 for HSA) and wide linear range response over 4 orders of magnitude. Moreover, there are very little interferences from common inorganic ions and other coexisting compounds. The developed method was successfully applied for detecting total protein in 50 human urine samples and the results were in good agreement with those reported by the hospital, which indicated a great potential for routine clinical application.

Graphical abstract: Inductively coupled plasma mass spectrometry for determination of total urinary protein with CdTe quantum dots label

Article information

Article type
Paper
Submitted
24 Jul 2011
Accepted
15 Sep 2011
First published
17 Okt 2011

J. Anal. At. Spectrom., 2011,26, 2493-2499

Inductively coupled plasma mass spectrometry for determination of total urinary protein with CdTe quantum dots label

Y. Tang, X. Jiao, R. Liu, L. Wu, L. Wu, X. Hou and Y. Lv, J. Anal. At. Spectrom., 2011, 26, 2493 DOI: 10.1039/C1JA10213C

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements