Issue 43, 2015

Generation of a pair of independently binding DNA aptamers in a single round of selection using proximity ligation

Abstract

The ability to rapidly generate a pair of aptamers that bind independently to a protein target would greatly extend their use as reagents for two site (‘sandwich’) assays. We describe here a method to achieve this through proximity ligation. Using lysozyme as a target we demonstrate that under optimal conditions such a pair of aptamers, with nanomolar affinities, can be generated in a single round.

Graphical abstract: Generation of a pair of independently binding DNA aptamers in a single round of selection using proximity ligation

Supplementary files

Article information

Article type
Communication
Submitted
19 Mär 2015
Accepted
24 Apr 2015
First published
27 Apr 2015

Chem. Commun., 2015,51, 9050-9053

Author version available

Generation of a pair of independently binding DNA aptamers in a single round of selection using proximity ligation

O. Chumphukam, T. T. Le, S. Piletsky and A. E. G. Cass, Chem. Commun., 2015, 51, 9050 DOI: 10.1039/C5CC02314A

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