Issue 25, 2015
From the journal:

Chemical Communications

Collagen labelling with an azide-proline chemical reporter in live cells†

Beatrice Amgarten,a   Rakesh Rajan,b   Nuria Martínez-Sáez,a   Bruno L. Oliveira,c   Inês S. Albuquerque,d   Roger A. Brooks,b   David G. Reid,a   Melinda J. Duera  and  Gonçalo J. L. Bernardes*ad  

* Corresponding authors

a Department of Chemistry, University of Cambridge, Lensfield Road, CB2 1EW Cambridge, UK
E-mail: gb453@cam.ac.uk

b Division of Trauma & Orthopaedic Surgery, University of Cambridge, Addenbrooke's Hospital, Cambridge CB2 0QQ, UK

c Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA, USA

d Instituto de Medicina Molecular, Faculdade de Medicina da Universidade de Lisboa, Av. Prof. Egas Moniz, 1649-028 Lisboa, Portugal
E-mail: gbernardes@medicina.ulisboa.pt
Web: http://gbernardes-lab.com

Abstract

We have developed a strategy for selective imaging of collagen in live foetal ovine osteoblasts. Our approach involves the incorporation of an azide-tagged proline in the biosynthesis of collagen followed by labelling using a strain-promoted [3+2] azide–alkyne cycloaddition reaction.

Graphical abstract: Collagen labelling with an azide-proline chemical reporter in live cells

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C4CC07974D
Article type
Communication
Submitted
08 Okt 2014
Accepted
17 Nov 2014
First published
18 Nov 2014
This article is Open Access
Creative Commons BY license

Chem. Commun., 2015,51, 5250-5252

Author version available

Download author version (PDF)

Permissions

Request permissions

Collagen labelling with an azide-proline chemical reporter in live cells

B. Amgarten, R. Rajan, N. Martínez-Sáez, B. L. Oliveira, I. S. Albuquerque, R. A. Brooks, D. G. Reid, M. J. Duer and G. J. L. Bernardes, Chem. Commun., 2015, 51, 5250 DOI: 10.1039/C4CC07974D

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements