Issue 2, 2022

A new metal-free near-infrared fluorescent probe based on nitrofuran for the detection and bioimaging of carbon monoxide releasing molecule-2 in vivo

Abstract

As a stable donor for releasing controlled amounts of CO, carbon monoxide releasing molecule-2 (CORM-2) is a new type of therapeutic drug that contributes to exploring the pathophysiological effects of CO. The accurate detection of CORM-2 in biological systems is of great significance for controlling its dosage as a therapeutic drug and elucidating the reaction mechanisms of CO, but currently there is a lack of metal-free near-infrared fluorescent probes. Herein, a new metal-free near-infrared fluorescent probe based on nitrofuran which could selectively identify CORM-2 was designed and it has been successfully applied in living cells, zebrafish and mice. After reacting with CORM-2, both the color and fluorescence signal of the solution are restored, which is ascribed to the reduction of the nitro group. The spectroscopic probe DXPN shows high sensitivity to CORM-2 with a low detection limit of 87 nM and near-infrared fluorescence emission of 712 nm. Notably, this is the first time that paper chips are being used as a carrier to detect CORM-2 through fluorescence signals instead of the traditional liquid phase detection mode of fluorescent probes. These superior properties of the probe make it a promising and reliable tool for exploring the role played by CORM-2 in biological systems.

Graphical abstract: A new metal-free near-infrared fluorescent probe based on nitrofuran for the detection and bioimaging of carbon monoxide releasing molecule-2 in vivo

Supplementary files

Article information

Article type
Paper
Submitted
28 sep 2021
Accepted
13 nov 2021
First published
02 dec 2021

Analyst, 2022,147, 268-273

A new metal-free near-infrared fluorescent probe based on nitrofuran for the detection and bioimaging of carbon monoxide releasing molecule-2 in vivo

X. Tang, Z. Li, Y. Li, Y. Zhang, Q. Wang and C. Zhang, Analyst, 2022, 147, 268 DOI: 10.1039/D1AN01766G

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements