Issue 11, 2021

A chondroitin sulfate based injectable hydrogel for delivery of stem cells in cartilage regeneration

Abstract

Chondroitin sulfate (CS), as a popular material for cartilage tissue engineering scaffolds, has been extensively studied and reported for its safety and excellent biocompatibility. However, the rapid degradation of pure CS scaffolds has brought a challenge to regenerate neo-tissue similar to natural articular cartilage effectively. Meanwhile, the poly(ethene glycol) (PEG) -based biopolymer is frequently applied as a structural constituent material because of its remarkable mechanical properties, long-lasting in vivo stability, and hypo-immunity. Here, we report that the combination of CS and hyperbranched multifunctional PEG copolymer (HB-PEG) could synergistically promote cartilage repair. The thiol functionalised CS (CS-SH)/HB-PEG hydrogel scaffolds were fabricated via thiol–ene reaction, which exhibits rapid gelation, excellent mechanical properties and prolonged degradation properties. We found that rat adipose-derived mesenchymal stem cells presented great cell viability and improved chondrogenesis in CS-SH/HB-PEG hydrogels. Moreover, the injectable hydrogel scaffolds reduced stem cell inflammatory response, consistent with the well-documented anti-inflammatory activities of CS.

Graphical abstract: A chondroitin sulfate based injectable hydrogel for delivery of stem cells in cartilage regeneration

Supplementary files

Article information

Article type
Paper
Submitted
28 mar 2021
Accepted
27 apr 2021
First published
27 apr 2021

Biomater. Sci., 2021,9, 4139-4148

A chondroitin sulfate based injectable hydrogel for delivery of stem cells in cartilage regeneration

X. Li, Q. Xu, M. Johnson, X. Wang, J. Lyu, Y. Li, S. McMahon, U. Greiser, S. A and W. Wang, Biomater. Sci., 2021, 9, 4139 DOI: 10.1039/D1BM00482D

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