Issue 39, 2024

Chemical control of CRISPR/Cpf1 editing via orthogonal activation and deactivation of crosslinked crRNA

Abstract

Through the integration of CRISPR/Cpf1 with optogenetics and a reduction-responsive motif, we have developed a photoactivatable cross-linked crRNA that enables precise genome editing upon light exposure. This system also allows for termination of editing activity through external application of reducing agent. The dual-stimuli-responsive CRISPR/Cpf1 editing process operates in a unique OFF → ON → OFF sequence, making it a valuable tool for investigating time-sensitive biological events.

Graphical abstract: Chemical control of CRISPR/Cpf1 editing via orthogonal activation and deactivation of crosslinked crRNA

Supplementary files

Article information

Article type
Communication
Submitted
08 mar 2024
Accepted
17 apr 2024
First published
18 apr 2024

Chem. Commun., 2024,60, 5197-5200

Chemical control of CRISPR/Cpf1 editing via orthogonal activation and deactivation of crosslinked crRNA

C. Lin, W. Chen, L. Liu and L. Cheng, Chem. Commun., 2024, 60, 5197 DOI: 10.1039/D4CC01106F

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