Issue 10, 2023

Optimization of precision nanofiber micelleplexes for DNA delivery

Abstract

As nucleic acid (NA) technologies continue to revolutionize medicine, new delivery vehicles are needed to effectively transport NA cargoes into cells. Uniform and length-tunable nanofiber micelleplexes have recently shown promise as versatile polymeric delivery vehicles for plasmid DNA, however the effects of several key parameters on micelleplex transfection and stability remain unknown. In this work, we compare poly(fluorenetrimethylenecarbonate)-b-poly(2-(dimethylamino)ethyl methacrylate) (PFTMC-b-PDMAEMA) nanofiber micelleplexes to nanosphere micelleplexes and PDMAEMA polyplexes, examining the effects of complexation buffer, the temporal and serum stability of nanofiber micelleplexes, as well as the effects of cell density, cell type, and polymer DPn upon transfection efficiency and cell viability. These studies are vital for understanding the formation and biological activity of micelleplexes in more detail and should inform the future design of more advanced polymeric NA delivery systems.

Graphical abstract: Optimization of precision nanofiber micelleplexes for DNA delivery

Supplementary files

Article information

Article type
Paper
Submitted
07 dec 2022
Accepted
03 mar 2023
First published
08 mar 2023

Biomater. Sci., 2023,11, 3512-3523

Optimization of precision nanofiber micelleplexes for DNA delivery

S. T. G. Street, H. C. Parkin, L. Shopperly, J. Chrenek, K. Letwin, S. M. Willerth and I. Manners, Biomater. Sci., 2023, 11, 3512 DOI: 10.1039/D2BM02014A

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