Issue 17, 2021

Three dimensionally printed nitrocellulose-based microfluidic platform for investigating the effect of oxygen gradient on cells

Abstract

In this article, we present a novel nitrocellulose-based microfluidic chip with 3-dimensional (3D) printing technology to study the effect of oxygen gradient on cells. Compared with conventional polydimethylsiloxane (PDMS) chips of oxygen gradient for cell cultures that can only rely on fluorescence microscope analysis, this hybrid nitrocellulose-based microfluidic platform can provide a variety of analysis methods for cells, including flow cytometry, western blot and RT-PCR, because the nitrocellulose-based chips with cells can be taken out from the growth chambers of 3D printed microfluidic chip and then used for cell collection or lysis. These advantages allow researchers to acquire more information and data on the basic biochemical and physiological processes of cell life. The effect of oxygen gradient on the zebrafish cells (ZF4) was used as a model to show the performance and application of our platform. Hypoxia caused the increase of intercellular reactive oxygen species (ROS) and accumulation of hypoxia-inducible factor 1α (HIF-1α). Hypoxia stimulated the transcription of hypoxia-responsive genes vascular endothelial growth factor (VEGF) and induced cell cycle arrest of ZF4 cells. The established platform is able to obtain more information from cells in response to different oxygen concentration, which has potential for analyzing the cells under a variety of pathological conditions.

Graphical abstract: Three dimensionally printed nitrocellulose-based microfluidic platform for investigating the effect of oxygen gradient on cells

Supplementary files

Article information

Article type
Paper
Submitted
25 maj 2021
Accepted
24 jul 2021
First published
29 jul 2021

Analyst, 2021,146, 5255-5263

Three dimensionally printed nitrocellulose-based microfluidic platform for investigating the effect of oxygen gradient on cells

P. Liu, L. Fu, Z. Song, M. Man, H. Yuan, X. Zheng, Q. Kang, D. Shen, J. Song, B. Li and L. Chen, Analyst, 2021, 146, 5255 DOI: 10.1039/D1AN00927C

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements